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Goat Anti-WISP1 Antibody (EB08178)

Related Products: EBP08178 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08178 Goat Anti-WISP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc
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EB08178 1
HEK293 overexpressing WISP1 (RC214390) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB08178 in the right panel (mock transfection in first lane in each panel), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
Official Symbol: WISP1
Accession Number(s): NP_003873.1; NP_543028.1
Human GeneID(s): 8840
Non-Human GeneID(s): 22402 (mouse), 65154 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).

Immunogen

Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1; NP_543028.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing WISP1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat,

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