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Goat Anti-WFDC2 (aa29-43) Antibody (EB09963)

Related Products: EBP09962 - Immunizing Peptide, EBP09963 - Immunizing Peptide, Goat Anti-WFDC2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09963 Goat Anti-WFDC2 (aa29-43) Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc
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EB09963 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed with EB09963 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB09963 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: dJ461P17.6, EDDM4, epididymal protein 4, epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, HE4, major epididy, MGC57529, WAP domain containing protein HE4-V4, WAP four-disulfide core domain 2, WAP5, WFDC2
Official Symbol: WFDC2
Accession Number(s): NP_006094.3
Human GeneID(s): 10406

Immunogen

Peptide with sequence GAEKTGVCPELQADQ, from the internal region of the protein sequence according to NP_006094.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human WFDC2 (myc and DYKDDDDK tagged), a band of approx. 25kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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