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Goat Anti-UHRF1 / ICBP90 Antibody (EB09807)

Related Products: EBP05943 - Immunizing Peptide, EBP09807 - Immunizing Peptide, Goat Anti-ICBP90 / UHRF1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09807 Goat Anti-UHRF1 / ICBP90 Antibody ELISA Allow up to 10 days for delivery 100µg specific antibody in 200µl $210.00 Aspiring

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ21925, hNP95, ICBP90, inverted CCAAT box-binding protein of 90 kDa, MGC138707, Np95, nuclear zinc finger protein Np95, RING finger protein 106, RNF106, transcription factor ICBP90, ubiquitin-like, containing PHD and RING finger domains, 1, UHRF1
Official Symbol: UHRF1
Accession Number(s): NP_001041666.1; NP_037414.3
Human GeneID(s): 29128
Non-Human GeneID(s): 316129 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001041666.1; NP_037414.3).

Immunogen

Peptide with sequence NKRTAEQSCDQKLTN, from the internal region of the protein sequence according to NP_001041666.1; NP_037414.3.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Preliminary experiments in HeLa, HEK293, HG2, Human Thymus lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Rat, Dog, Cow

Background Reference

Arita K, Ariyoshi M, Tochio H, Nakamura Y, Shirakawa M.
Recognition of hemi-methylated DNA by the SRA protein UHRF1 by a base flipping mechanism.
Nature. 2008 Oct 9;455(7214):818-21. Epub 2008 Sep 3.
PMID: 18772891

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