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Code Name Applications Availability Size Tested species Price Grade  
EB08515 Goat Anti-TRPV5 Antibody Pep-ELISA, WB, ICC, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB08515 1
EB08515 (1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPV5, transient receptor potential cation channel, subfamily V, member 5, CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3
Official Symbol: TRPV5
Accession Number(s): NP_062815.2
Human GeneID(s): 56302
Non-Human GeneID(s): 194352 (mouse), 116469 (rat)

Immunogen

Peptide with sequence C-SHRGWEILRQNT, from the internal region of the protein sequence according to NP_062815.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3µg/ml.

Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for human retinal pigment epithelium as described in the following paper: Kennedy et al, Mol Vis. 2010 Apr 14;16:665-75; PMID: 20405023.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody has been successfully used in IF in Human:
Tomilin VN, Cherezova AL, Negulyaev YA, Semenova SB.
TRPV5/V6 Channels Mediate Ca(2+) Influx in Jurkat T Cells Under the Control of Extracellular pH.
J Cell Biochem. 2016 Jan;117(1):197-206
PMID: 26096460

This antibody has been successfully used in WB and ICC in Human:
Kennedy BG, Torabi AJ, Kurzawa R, Echtenkamp SF, Mangini NJ.
Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium.
Mol Vis. 2010 Apr 14;16:665-75.
PMID: 20405023

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