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Goat Anti-STAT3 (isoform 1 and 2) Antibody (EB05073)

Related Products: EBP05073 - Immunizing Peptide, EBP07379 - Immunizing Peptide, Goat Anti-STAT3 Antibody, Goat Anti-STAT3 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05073 Goat Anti-STAT3 (isoform 1 and 2) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Mouse $315.00 Elite

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EB05073 1
EB05073 (4µg/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
Official Symbol: STAT3
Accession Number(s): NP_003141.2; NP_644805.1
Human GeneID(s): 6774
Non-Human GeneID(s): 20848 (mouse), 25125 (rat)
Important Comments: This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)

Immunogen

Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2; NP_644805.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Mouse Thymus shows preferential staining of the Medulla over the Cortex. Recommended concentration: 4-8µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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FAQs

In which mouse tissues / cell lysates has the antibody been tested?

The antibody stained mouse thymic medulla tissue in IHC assays. In WB assays the antibody was tested in NIH3T3 lysates.

 

In which concentration did you use your antibody?

WB assays: 1µg/ml

IHC assays: 10µg/ml