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Code Name Applications Availability Size Tested species Price Grade  
EB07208-T Goat Anti-SOD1 Antibody - Trial Pep-ELISA, WB, EIA Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

Ordering Information for

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$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

TestShot
EB07208-T 1
EB07208 (0.02µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07208-T 2
EB07208 (0.01µg/ml) staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07208-T 3
EB07208 (1.5ug/ml) as the reporter with EB002010 as the capture rabbit antibody (5ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)), HGNC:11179, ALS, ALS1, IPOA, SOD, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic, HEL-S-44, hSod1, epididymis secretory protein Li 44
Official Symbol: SOD1
Accession Number(s): NP_000445.1
Human GeneID(s): 6647
Non-Human GeneID(s): 20655 (mouse), 24786 (rat)

Immunogen

Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 18kDa band observed in Mouse Brain lysates, in purified membranes of cell line HeLa and in lysates of NIH3T3. Approx 20kDa observed in HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to human NP_000445.1, mouse NP_035564.1 and rat NP_058746.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Frazziano G, Al Ghouleh I, Baust J, Shiva S, Champion HC, Pagano PJ.
Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4.
Am J Physiol Heart Circ Physiol. 2014 Jan 15;306(2):H197-205.
PMID: 24213612

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