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Goat Anti-SNX26 / TCGAP (C Terminus) Antibody (EB05916)

Code Name Applications Availability Size Tested species Price Grade  
EB05916 Goat Anti-SNX26 / TCGAP (C Terminus) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB05916 1
EB05916 (2.5µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein, NOMA-GAP, neurite outgrowth multiadaptor RhoGAP protein
Official Symbol: SNX26
Accession Number(s): NP_443180.2
Human GeneID(s): 115703
Non-Human GeneID(s): 233071 (mouse)

Immunogen

Peptide with sequence SWSLHSEGQTRSYC, from the C Terminus of the protein sequence according to NP_443180.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 75kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1µg/ml antibody staining. EB05916 may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently we cannot find a precise explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration, 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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