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Code Name Applications Availability Size Tested species Price Grade  
EB11540 Goat Anti-RIF1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11540 1
EB11540 (1µg/ml) staining of HeLa and Mouse D3 (ES) lysates (35µg protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKFZp434D1026, DKFZp781N1478, FLJ12870, OTTHUMP00000162712, OTTHUMP00000207206, OTTHUMP00000207207, OTTHUMP00000207208, OTTHUMP00000207210, RAP1 interacting factor homolog (yeast), rap1-interacting factor 1 homolog, RIF1, telomere-associated protein RIF1
Official Symbol: RIF1
Accession Number(s): NP_060621.3; NP_001171134.1
Human GeneID(s): 55183
Non-Human GeneID(s): 51869 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Immunogen

Peptide with sequence C-RNLQSRWRSPSHEN, from the C Terminus of the protein sequence according to NP_060621.3; NP_001171134.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 270kDa band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kDa according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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