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Goat Anti-PNPLA3 / Adiponutrin Antibody (EB08402)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB08402 | Goat Anti-PNPLA3 / Adiponutrin Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrinOfficial Symbol: PNPLA3
Accession Number(s): NP_473429.2
Human GeneID(s): 80339
Non-Human GeneID(s): 116939 (mouse), 362972 (rat)
Important Comments: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
Immunogen
Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 45kDa band observed in Mouse Heart lysates and in Mouse and Rat Adrenal gland lysates (calculated MW of 42.6kDa according to Mouse NP_473429.2 and 47.1kDa according to Rat NP_001269253.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 15kDa in the Adrenal Gland lysates. This band was successfully blocked by incubation with the immunizing peptide. Preliminary testing was unsuccessful on Human Heart for this particular batch.
IHC: In paraffin embedded Human Skin shows membranous staining of basak kayer cells. Recommended concentration: 4-6µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat
Specific References
This antibody has been successfully used in Western blot on Mouse:
Kumari M, Schoiswohl G, Chitraju C, Paar M, Cornaciu I, Rangrez AY, Wongsiriroj N, Nagy HM, Ivanova PT, Scott SA, Knittelfelder O, Rechberger GN, Birner-Gruenberger R, Eder S, Brown HA, Haemmerle G, Oberer M, Lass A, Kershaw EE, Zimmermann R, Zechner R.
Adiponutrin functions as a nutritionally regulated
lysophosphatidic acid acyltransferase.
Cell Metab. 2012 May 2;15(5):691-702.
PMID: 22560221
This antibody has been successfully used in Western blot on Rat:
Gustavsson C, Soga T, Wahlström E, Vesterlund M, Azimi A, Norstedt G, Tollet-Egnell P.
Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic
fatty rats.
J Mol Endocrinol. 2011 Aug 25;47(2):129-43.
PMID: 21673048