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Goat Anti-PIR51 / RAD51AP1 Antibody (EB05691)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05691 | Goat Anti-PIR51 / RAD51AP1 Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: RAD51AP1, PIR51, RAD51-interacting protein, RAD51 associated protein 1Official Symbol: RAD51AP1
Accession Number(s): NP_001124334.1; NP_006470
Human GeneID(s): 10635
Important Comments: This antibody is expected to recognise both reported isoforms.
Immunogen
Peptide with sequence C-ARVKPLHPNATST, from the C Terminus of the protein sequence according to NP_001124334.1; NP_006470.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Dog, Cow