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Goat Anti-PDCD1 Antibody (EB06753)

Code Name Applications Availability Size Tested species Price Grade  
EB06753 Goat Anti-PDCD1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]
Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB06753 1
EB06753 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDCD1, programmed cell death 1, HGNC:8760, PD1, SLEB2, hPD-1, hPD-l, programmed death 1, CD279
Official Symbol: PDCD1
Accession Number(s): NP_005009.2
Human GeneID(s): 5133

Immunogen

Peptide with sequence C-GELDFQWREKTPE, from the internal region of the protein sequence according to NP_005009.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in Human Bone Marrow lysates (calculated MW of 31.7kDa according to NP_005009.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in Western blot on Human:
Guozhi Xia, Xiaopu Zheng, Xinye Yao, Xiaowei Yao, Zhongwei Liu, Junkui Wang.
Expression of programmed cell death-1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy.
Clin Cardiol. 2016 Dec 27.
PMID: 28026044

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