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Code Name Applications Availability Size Tested species Price Grade  
EB06776 Goat Anti-Parvalbumin Antibody Pep-ELISA, WB, FC, IHC Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06776 1
EB06776 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer).r. Detected by chemiluminescence.
EB06776 2
EB06776 (0.15µg/ml) staining of adenovirus-mediated gene transfer in Rat Heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected Rat Skeletal Muscle. Detected by chemiluminescence.
EB06776 3
EB06776 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06776 4
EB06776 (2.5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759
Official Symbol: PVALB
Accession Number(s): NP_002845.1
Human GeneID(s): 5816
Important Comments: Reported variants represent identical protein: NP_002845.1, NP_001302461.1

Immunogen

Peptide with sequence C-GVDEFSTLVAES, from the C Terminus of the protein sequence according to NP_002845.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5µg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat

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