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Goat Anti-Pancreatic Polypeptide / PPY Antibody (EB06805)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06805 | Goat Anti-Pancreatic Polypeptide / PPY Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide YOfficial Symbol: PPY
Accession Number(s): NP_002713.1
Human GeneID(s): 5539
Immunogen
Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 35-38kDa band in Human Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3µg/ml. Paraffin embedded Human Pancreas and Intestine. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog
Specific References
This antibody has been successfully used in IF on Rat:
Md Moin AS, Dhawan S, Cory M, Butler PC, Rizza RA, Butler AE.
Increased Frequency of Hormone Negative
and Polyhormonal Endocrine Cells in Lean
Individuals With Type 2 Diabetes.
J Clin Endocrinol Metab. 2016 Oct;101(10)
PMID: 27472443
This antibody has been successfully used in IHC on Human:
Butler AE, Dhawan S, Hoang J, Cory M, Zeng K, Fritsch H, Meier JJ, Rizza RA, Butler PC.
β-Cell Deficit in Obese Type 2 Diabetes, a Minor Role of β-Cell Dedifferentiation and Degranulation.
J Clin Endocrinol Metab. 2016 Feb;101(2):523-32.
PMID: 26700560
This antibody has been successfully used in Western blot on Human:
F. Aragón, M. Karaca, A. Novials, R.Maldonadoa, P. Maechler, and B. Rubí.
Pancreatic polypeptide regulates glucagon release through PPYR1 receptors expressed in mouse and human alpha-cells.
Biochim Biophys Acta. 2015 Feb;1850(2):343-51.
PMID: 25445712