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Goat Anti-NRAS Antibody (EB08365)

Related Products: EBP08365 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08365 Goat Anti-NRAS Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

Ordering Information for

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$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) 
Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08365 1
EB08365 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08365 2
EB08365 (0.1µg/ml) staining of Rat Intestines lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08365 3
EB08365 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NRAS, neuroblastoma RAS viral (v-ras) oncogene homolog, N-ras, NRAS1, N-ras protein part 4, v-ras neuroblastoma RAS viral oncogene homolog
Official Symbol: NRAS
Accession Number(s): NP_002515.1
Human GeneID(s): 4893
Non-Human GeneID(s): 18176 (mouse), 24605 (rat)

Immunogen

Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.
IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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