Change country
Home

Goat Anti-NCAM2 / OCAM Antibody (EB06991)

Related Products: EBP06991 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06991 Goat Anti-NCAM2 / OCAM Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

Order direct from Everest

$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) 
Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06991 1
EB06991 (0.5µg/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from customer.
EB06991 2
EB06991 (0.5µg/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from customer.
EB06991 3
EB06991 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NCAM2, neural cell adhesion molecule 2, HGNC:7657, MGC51008, NCAM21, OCAM
Official Symbol: NCAM2
Accession Number(s): NP_004531.2
Human GeneID(s): 4685
Non-Human GeneID(s): 17968 (mouse), 288280 (rat)

Immunogen

Peptide with sequence C-KVSNDIIQSKEDDSK, from the C Terminus of the protein sequence according to NP_004531.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.
IHC: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration: 0.5µg/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Product Reviews

Please login to review this product.