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Goat Anti-MID2 / TRIM1 Antibody (EB05823)

Related Products: EBP05823 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05823 Goat Anti-MID2 / TRIM1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
www.arp1.com
EB05823 1
EB05823 (0.1µg/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05823 2
EB05823 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MID2, TRIM1, midline 2, FXY2, midin 2, tripartite motif protein 1, RP6-191P20.2, FLJ37715, FLJ41813, FXY2, RNF60
Official Symbol: MID2
Accession Number(s): NP_036348.2; NP_438112.2
Human GeneID(s): 11043
Non-Human GeneID(s): 23947 (mouse)
Important Comments: This antibody is expected to recognise both human isoforms of this protein.

Immunogen

Peptide with sequence QESPYVSGMKTCH, from the C Terminus of the protein sequence according to NP_036348.2; NP_438112.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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