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Goat Anti-LXR alpha / LXR beta Antibody - Trial (EB06522-T)

Code Name Applications Availability Size Tested species Price Grade  
EB06522-T Goat Anti-LXR alpha / LXR beta Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

TestShot
EB06522-T 1
EB06522 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LXRA, LXR-a, RLD-1, NR1H3, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor, OTTHUMP00000198038, liver X receptor alpha, liver X receptor-alpha, LXRB, LX receptor beta, iver X receptor beta, liver X receptor-beta, nuclear orphan receptor LXR-beta, oxysterols receptor LXR-beta
Official Symbol: NR1H3; NR1H2
Accession Number(s): NP_005684.2; NP_009052.3
Human GeneID(s): 10062 , 7376
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3).

Immunogen

Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2; NP_009052.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Human Spleen lysates (calculated MW of 50.5kDa according to NP_005684.2 and 50.1kDa according to NP_009052.3). Recommended concentration: 1-3µg/ml. Additional minor bands were also consistently observed in Human Spleen at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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