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Goat Anti-INADL / PATJ Antibody (EB06197)

Code Name Applications Availability Size Tested species Price Grade  
EB06197 Goat Anti-INADL / PATJ Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB06197 1
EB06197 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: INADL, PATJ, PDZ domain protein (Drosophila inaD-like), protein associated to tight junctions, InaD-like (Drosophila), RP4-537K17.1, Cipp, FLJ26982, InaD-like, InaD-like, PALS1-associated tight junction protein, PDZ domain protein, channel-interacting PDZ domain protein, inactivation no after-potential D-like protein
Official Symbol: INADL
Accession Number(s): NP_795352.2
Human GeneID(s): 10207

Immunogen

Peptide with sequence PENPATDKLQVLQ-C, from the N Terminus of the protein sequence according to NP_795352.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human Brain and Kidney lysates consistently showed a strong band at 100kDa followed by bands at ~55kDa, 45kDa and 40kDa at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of approx. 196Kda according to NP_795352.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Is this protein particularly susceptible to proteolysis?
IHC: In paraffin embedded Human Testis shows strong staining of spermatids. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

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