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Goat Anti-HOXD10 Antibody (EB08884)

Related Products: EBP08884 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08884 Goat Anti-HOXD10 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) 
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08884 1
EB08884 (0.2µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08884 2
EB08884 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10
Official Symbol: HOXD10
Accession Number(s): NP_002139.2
Human GeneID(s): 3236
Non-Human GeneID(s): 15430 (mouse), 303991 (rat)

Immunogen

Peptide with sequence PNRSCRIEQPVTQQ, from the internal region of the protein sequence according to NP_002139.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 37 kDa band observed in Human Kidney lysates (calculated MW of 38.4 kDa according to NP_002139.2). Recommended concentration: 0.2-0.6µg/ml. Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows nuclear staining in some epithelial cells of PCT. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Horse, Cow

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