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Goat Anti-Histamine Receptor H2 Antibody (EB06905)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06905 | Goat Anti-Histamine Receptor H2 Antibody | Pep-ELISA, IHC, IF |  |
100µg specific antibody in 200µl | Rat | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242Official Symbol: HRH2
Accession Number(s): NP_071640.1; NP_001124527.1
Human GeneID(s): 3274
Important Comments: This antibody is expected to recognise both reported isoforms.
Immunogen
Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1; NP_001124527.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Preliminary experiments gave bands at approx 55kDa and a stronger 28kDa in Human Colon and Duodenum lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 40.1kDa according to NP_071640.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Immunofluorescence:This antibody was successfully used on coronal rat brain slices as reported in Peng et al, Neurosci Lett. 2013 Apr 29;541:87-92. PMID:23466693.
Species Reactivity
Tested: RatExpected from sequence similarity: Human, Mouse, Rat, Dog
Specific References
This antibody has been successfully used in IF on Rat:
Bin Li, Xiao-Yang Zhang, Ai-Hong Yang, Xiao-Chun Peng, Zhang-Peng Chen, Jia-Yuan Zhou, Ying-Shing Chan, Jian-Jun Wang and Jing-Ning Zhu.
Histamine Increases Neuronal Excitability and Sensitivity of the Lateral Vestibular Nucleus and Promotes Motor Behaviors via HCN Channel Coupled to H2 Receptor.
Front Cell Neurosci. 2017 Jan 10;10:300.
PMID: 28119568
This antibody has been successfully used in IF on Rat:
Zhang J, Zhuang QX, Li B, Wu GY, Yung WH, Zhu JN, Wang JJ.
Selective Modulation of Histaminergic Inputs on Projection Neurons of Cerebellum Rapidly Promotes Motor Coordination via HCN Channels.
Mol Neurobiol. 2016 Mar;53(2):1386-401.
PMID: 25633097
This antibody has been successfully used in IF on Rat:
Zhang XY, Yu L, Zhuang QX, Peng SY, Zhu JN, Wang JJ.
Postsynaptic mechanisms underlying the excitatory action of histamine on medial vestibular nucleus neurons in rats.
Br J Pharmacol. 2013 Sep;170(1):156-69.
PMID: 23713466
This antibody has been successfully used in IF on Rat:
Peng SY, Zhuang QX, He YC, Zhu JN, Wang JJ.
Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors.
Neurosci Lett. 2013 Apr 29;541:87-92.
PMID: 23466693