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Goat Anti-Histamine Receptor H1 (C Term) Antibody (EB06904)

Related Products: EBP06904 - Immunizing Peptide, EBP07699 - Immunizing Peptide, Goat Anti-Histamine receptor H1(internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06904 Goat Anti-Histamine Receptor H1 (C Term) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06904 1
EB06904 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06904 2
EB06904 (4µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH1, histamine receptor, subclass H1, OTTHUMP00000160133, histamine H(1) receptor
Official Symbol: HRH1
Accession Number(s): NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1
Human GeneID(s): 3269
Non-Human GeneID(s): 15465 (mouse), 24448 (rat)
Important Comments: Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.

Immunogen

Peptide with sequence CNENFKKTFKRILH, from the C Terminus of the protein sequence according to NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).
IHC: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6µg/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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