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Goat Anti-HIP-55 / SH3P7 Antibody (EB05432)

Related Products: EBP05432 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05432 Goat Anti-HIP-55 / SH3P7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) 
Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05432 1
EB05432 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05432 2
EB05432 (1µg/ml) staining of 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DBNL, HIP-55, SH3P7, src homology 3 domain-containing protein HIP-55, HIP-55 protein, drebrin F, drebrin-like, ABP1
Official Symbol: DBNL
Accession Number(s): NP_054782.2; NP_001014436.1; NP_001116428.1
Human GeneID(s): 28988
Non-Human GeneID(s): 13169 (mouse), 83527 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1)

Immunogen

Peptide with sequence AANLSRNGPALQE-C, from the N Terminus of the protein sequence according to NP_054782.2; NP_001014436.1; NP_001116428.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Jurkat and 3T3 lysates (calculated MW of 49.0kDa according to Human NP_001116428.1 and 48.7kDa according to Mouse NP_001139780.1). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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