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Code Name Applications Availability Size Tested species Price Grade  
EB08896 Goat Anti-FURIN / PCSK3 Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc
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EB08896 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with EB08896 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB08896 (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FURIN, furin (paired basic amino acid cleaving enzyme), FUR, PACE, PCSK3, SPC1, FES upstream region, dibasic processing enzyme, furin, furin, membrane associated receptor protein, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein), proprotein convertase subtilisin/kexin type 3
Official Symbol: FURIN
Accession Number(s): NP_002560.1
Human GeneID(s): 5045

Immunogen

Peptide with sequence C-RTKRDVYQEPTDP, from the internal region of the protein sequence according to NP_002560.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx. 110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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