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Code Name Applications Availability Size Tested species Price Grade  
EB06507 Goat Anti-FHL1 / SLIM1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06507 1
EB06507 staining (0.05µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06507 2
EB06507 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FHL1, SLIM1, KYO-T, RP11-535K18.1, FHL1A, four and a half LIM domains 1, Four-and-a-half LIM domains 1, skeletal muscle LIM-protein1, FHL1B, KYOT, MGC111107, SLIMMER, XMPMA, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, FHL-1, FLH1A, SLIM-1, LIM protein SLIMMER, SLIM
Official Symbol: FHL1
Accession Number(s): NP_001440.2; NP_001153171.1
Human GeneID(s): 2273
Non-Human GeneID(s): 14199 (mouse), 25177 (rat)
Important Comments: This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Immunogen

Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.2; NP_001153171.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440). Recommended concentration: 0.05-0.3µg/ml.
IHC: In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells.. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in Mouse in the following paper:
Tang T, Lai NC, Hammond HK, Roth DM, Yang Y, Guo T, Gao MH.
Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice.
J Am Coll Cardiol. 2010 Apr 6;55(14):1476-86.
PMID: 20359598

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