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Goat Anti-FAM62A Antibody (EB08321)

Related Products: EBP08321 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08321 Goat Anti-FAM62A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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Email: [email protected]
www.arp1.com
EB08321 1
EB08321 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08321 2
HEK293 overexpressing FAM62A (RC201380) and probed with EB08321 (mock transfection in first lane), tested by Origene.
EB08321 3
EB08321 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FAM62A, family with sequence similarity 62 (C2 domain containing), member A, KIAA0747, MBC2, membrane bound C2 domain containing protein, vp115
Official Symbol: FAM62A
Accession Number(s): NP_056107.1
Human GeneID(s): 23344
Non-Human GeneID(s): 23943 (mouse), 29579 (rat)

Immunogen

Peptide with sequence C-QKKRTLSPEFNER, from the internal region of the protein sequence according to NP_056107.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 150kDa band observed in Human Adipose lysates (calculated MW of 123kDa according to NP_056107.1). In transfected HEK293 transiently expressing FAM62A a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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