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Goat Anti-DLC1 (Isoforms 1 and 3) Antibody (EB06436)

Related Products: EBP06436 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06436 Goat Anti-DLC1 (Isoforms 1 and 3) Antibody Pep-ELISA, IHC, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB06436 1
EB06436 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, deleted in liver cancer 1, Rho-GTPase-activating protein 7, START domain containing protein 12, StAR-related lipid transfer protein 12, StAR-related lipid transfer (START) domain containing 12, deleted in liver cancer 1 variant 2
Official Symbol: DLC1
Accession Number(s): NP_872584.2; NP_079043.3
Human GeneID(s): 10395
Non-Human GeneID(s): 50768 (mouse)
Important Comments: This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).

Immunogen

Peptide with sequence SVAIRKRSWEEHC, from the N Terminus of the protein sequence according to NP_872584.2; NP_079043.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: In paraffin embedded Human Adrenal Gland (Medulla) shows strong reticulate staining in the cytoplasm of medular cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Pig

Specific Reference

This antibody has been successfully used in WB in Human:
Ko FC, Yeung YS, Wong CM, Chan LK, Poon RT, Ng IO, Yam JW.
Deleted in liver cancer 1 isoforms are distinctly expressed in human tissues, functionally different and under differential transcriptional regulation in hepatocellular carcinoma.
Liver Int. 2010 Jan;30(1):139-48.
PMID: 19874489

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