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Goat Anti-DKK3 / REIC Antibody (EB05361)

Related Products: EBP05361 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05361 Goat Anti-DKK3 / REIC Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05361 1
EB05361 staining (1µg/ml) of HEK293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by chemiluminescence.
EB05361 2
EB05361 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKK3, REIC, dickkopf homolog 3 (Xenopus laevis), DKK-3, RIG-like 5-6, RIG-like 7-1, dickkopf 3, Dickkopf gene 3, dickkopf (Xenopus laevis) homolog 3, dickkopf homolog 3, RIG, regulated in glioma
Official Symbol: DKK3
Accession Number(s): NP_037385.2; NP_056965.3; NP_001018067.1
Human GeneID(s): 27122
Important Comments: NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.

Immunogen

Peptide with sequence C-AAAALLGGEEI, from the C Terminus of the protein sequence according to NP_037385.2; NP_056965.3; NP_001018067.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 70kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1;238(2):301-13.; PMID: 10570958 ). Recommended concentration: 0.03-0.1µg/ml.
IHC: In paraffin embedded Human Brain Cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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