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Goat Anti-DCTN3 Antibody (EB10446)

Related Products: EBP10445 - Immunizing Peptide, EBP10446 - Immunizing Peptide, Goat Anti-DCTN3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10446 Goat Anti-DCTN3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

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$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) 
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10446 1
EB10446 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10446 2
EB10446 (0.2µg/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCTN22, DCTN-22, dynactin 3, dynactin 3 (p22), dynactin 3, isoform 1, dynactin light chain, MGC111190, DCTN3
Official Symbol: DCTN3
Accession Number(s): NP_009165.1; NP_077324.1
Human GeneID(s): 11258
Non-Human GeneID(s): 53598 (mouse), 362504 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).

Immunogen

Peptide with sequence C-QVALGNISSKRE, from the internal region of the protein sequence according to NP_009165.1; NP_077324.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 20kDa band observed in Human Kidney, Skeletal Muscle and Pancreas lysates (calculated MW of 19.5kDa according to NP_077324.1). In addition, a 25kDa band is observed in both Mouse and Rat Skeletal Muscle lysates accompanied by an 18kDa band in Mouse Skeletal Msuscle. In Mouse the protein is known as p24. The 18kDa band could reprsent a smaller isoform. Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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