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Goat Anti-DAP10 / HCST Antibody (EB07489)

Related Products: EBP07489 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07489 Goat Anti-DAP10 / HCST Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB07489 1
EB07489 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HCST, DAP10, hematopoietic cell signal transducer, KAP10, PIK3AP, DNAX-activation protein 10, phosphoinositide-3-kinase adaptor protein
Official Symbol: HCST
Accession Number(s): NP_055081.1; NP_001007470.1
Human GeneID(s): 10870
Non-Human GeneID(s): 23900 (mouse), 474146 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).

Immunogen

Peptide with sequence C-RSPAQEDGKVYIN, from the C Terminus of the protein sequence according to NP_055081.1; NP_001007470.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in human spleen lysates (calculated MW of 9.5kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30;285(5428):730-2.; PMID: 10426994). Recommended concentration: 0.2-0.6µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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