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Goat Anti-Coronin 1 / TACO Antibody (EB06057)

Related Products: EBP06057 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06057 Goat Anti-Coronin 1 / TACO Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB06057 1
EB06057 (0.3µg/ml) staining of Human Lymphoblastoid (RIPA buffer, (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06057 2
HEK293 overexpressing CORO1A (RC210753) and probed with EB06057 (mock transfection in first lane), tested by Origene.
EB06057 3
EB06057 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06057 4
EB06057 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPINA, Clabp TACO, coronin, actin binding protein, 1A, coronin-1, coronin, actin-binding, 1A, coronin, actin-binding protein, 1A, FLJ41407, MGC117380
Official Symbol: CORO1A
Accession Number(s): NP_009005.1
Human GeneID(s): 11151
Non-Human GeneID(s): 12721 (mouse), 155151 (rat)

Immunogen

Peptide with sequence C-KRLDRLEETVQAK, from the C Terminus of the protein sequence according to NP_009005.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in Western blot in Mouse:
Honoré B, Buus S, Claësson MH.
Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.
Proteome Sci. 2008 Jun 10;6:18.
PMID: 18544163

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