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Goat Anti-Cdc42-binding kinase alpha Antibody (EB06363)

Related Products: EBP06363 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06363 Goat Anti-Cdc42-binding kinase alpha Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06363 1
EB06363 (3.8µg/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDC42BPA, MRCK, MRCKA, PK428, FLJ23347, KIAA0451, CDC42 binding protein kinase alpha (DMPK-like), ser-thr protein kinase PK428, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Ser-Thr protein kinase related to the myotonic dystrophy protein kinase, DKFZp686L1738, CDC42 binding protein kinase beta
Official Symbol: CDC42BPA
Accession Number(s): NP_055641.3; NP_003598.2
Human GeneID(s): 8476
Non-Human GeneID(s): 226751 (mouse), 114116 (rat)
Important Comments: This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).

Immunogen

Peptide with sequence SGEVRLRQLEQFI-C, from the N Terminus of the protein sequence according to NP_055641.3; NP_003598.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Cerebellum shows cytoplasm staining in purkinje cells. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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