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Goat Anti-CCKBR Antibody (EB06767)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06767 | Goat Anti-CCKBR Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: CCKBR, cholecystokinin B receptor, HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor, CCK2ROfficial Symbol: CCKBR
Accession Number(s): NP_795344.1
Human GeneID(s): 887
Non-Human GeneID(s): 12426 (mouse), 25706 (rat)
Immunogen
Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1 and 49.0kDa according to Rat NP_037297.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 5µg/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Cow
Specific References
This antibody has been successfully used in IHC in Human:
Quattrone A, Dewaele B, Wozniak A, Bauters M, Vanspauwen V, Floris G, Schöffski P, Chibon F, Coindre JM, Sciot R, Debiec-Rychter M.
Promoting role of cholecystokinin 2 receptor (CCK2R) in gastrointestinal stromal tumours pathogenesis.
J Pathol. 2012 Jul 11. doi: 10.1002/path.4071.
PMID: 22786615
This antibody has been successfully used in Western blot in Rat:
El-Kouhen K, Morisset J.
Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells.
Int J Endocrinol. 2009;2009:875167.
PMID: 20011057
This antibody has been successfully used in ICC/IF in Rat:
Berna MJ, Seiz O, Nast JF, Benten D, Bläker M, Koch J, Lohse AW, Pace A.
CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production.
J Biol Chem. 2010 Dec 10;285(50):38905-14.
PMID: 20843811
This antibody has been successfully used in ICC/IF in Rat:
El-Kouhen K, Morisset J.
Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line.
Pancreas. 2010 Mar;39(2):127-34.
PMID: 19959964