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Code Name Applications Availability Size Tested species Price Grade  
EB06957 Goat Anti-CBX5 / HP1-Alpha Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06957 1
EB06957 (0.1µg/ml) staining of K562 (A), MCF7 (B) and NIH3T3 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06957 2
EB06957 (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06957 3
EB06957 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha, chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha
Official Symbol: CBX5
Accession Number(s): NP_036249.1; NP_001120793.1; NP_001120794.1
Human GeneID(s): 23468
Important Comments: Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.

Immunogen

Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1; NP_001120793.1; NP_001120794.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

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