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Goat Anti-calbindin D28 Antibody (EB11759)

Related Products: EBP11759 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11759 Goat Anti-calbindin D28 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB11759 1
EB11759 (0.1µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
EB11759 2
EB11759 (0.03µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11759 3
EB11759 (0.1µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CALB, CALB1, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, calbindin D28, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, vitamin D-dependent calcium-binding protein, avian-type
Official Symbol: CALB1
Accession Number(s): NP_004920.1
Human GeneID(s): 793
Non-Human GeneID(s): 83839 (rat)
Important Comments: Immunizing peptide overlaps EF hand 2, but not any calcium binding site.

Immunogen

Peptide with sequence C-KTFVDQYGQRDDGK, from the internal region of the protein sequence according to NP_004920.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519..Recommended concentration: 0.03-0.1µg/ml.
IHC: Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration, 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow

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