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Goat Anti-CACNB4 (C terminus) Antibody (EB06591)

Code Name Applications Availability Size Tested species Price Grade  
EB06591 Goat Anti-CACNB4 (C terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc
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Waltham, MA 02452
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EB06591 1
EB06591 (0.2µg/ml) staining of human bone marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06591 2
HEK293 overexpressing CACNB4 (RC210440) and probed with EB06591 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: CACNB4, CAB4, CACNLB4, calcium channel, voltage-dependent, beta 4 subunit, dihydropyridine-sensitive L-type, calcium channel beta-4 subunit
Official Symbol: CACNB4
Accession Number(s): NP_001005747.1; NP_000717.2; NP_001005746.1
Human GeneID(s): 785
Non-Human GeneID(s): 12298 (mouse), 58942 (rat)
Important Comments: This antibody is expected to recognise all reported protein isoforms of human CACNB4 (NP_001005747.1; NP_000717.2; NP_001005746.1; )

Immunogen

Peptide with sequence CSPGGYSHDSRHRL, from the C Terminus of the protein sequence according to NP_001005747.1; NP_000717.2; NP_001005746.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 60+55kDa bands observed in Human Bone Marrow lysates and in transfected HEK293 transiently expressing CACNB4 (calculated MW of 58.2kDa according to NP_000717.2 and 54.7kDa according to NP_001005747.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

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