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Goat Anti-Biglycan Preproprotein Antibody (EB07505)

Code Name Applications Availability Size Tested species Price Grade  
EB07505 Goat Anti-Biglycan Preproprotein Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB07505 1
EB07505 (0.1µg/ml) staining of Rat Skin lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Biglycan Preproprotein, biglycan, BGN, DSPG1, PG-S1, PGI, SLRR1A, bone/cartilage proteoglycan-I, dermatan sulphate proteoglycan I, small leucine-rich protein 1A
Official Symbol: BGN
Accession Number(s): NP_001702.1
Human GeneID(s): 633
Non-Human GeneID(s): 12111 (mouse), 25181 (rat)

Immunogen

Peptide with sequence C-DRLAIQFGNYKK, from the C Terminus of the protein sequence according to NP_001702.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 37kDa band observed in Rat Skin lysates (calculated MW of 41.7kDa according to Human NP_001702.1 and Rat NP_058783.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in IHC in Human:
Burssens A, Forsyth R, Bongaerts W, Jagodzinski M, Mahieu N, Praet M, Victor J.
Arguments for an increasing differentiation towards fibrocartilaginous components in midportion Achilles tendinopathy.
Knee Surg Sports Traumatol Arthrosc. 2012 Sep 23.
PMID: 23001016

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