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Goat Anti-BAF53A and BAF53B Antibody (EB06344)

Related Products: EBP06344 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06344 Goat Anti-BAF53A and BAF53B Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06344 1
EB06344 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, Arp4, INO80K, MGC5382, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 complex subunit K, actin-related protein 4, hArpN beta
Official Symbol: ACTL6A / ACTL6B
Accession Number(s): NP_004292.1; NP_817126.1; NP_057272.1
Human GeneID(s): 51412 , 86
Important Comments: This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).

Immunogen

Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1; NP_817126.1; NP_057272.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 48kDa band observed in Hela lysates (predicted MW of 49kDa according to NP_057272, NP_004292). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow,

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