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Goat Anti-ATP6IP2 / Renin receptor Antibody (EB06118)

Related Products: EBP06118 - Immunizing Peptide, Goat Anti-ATP6IP2 / Renin receptor Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06118 Goat Anti-ATP6IP2 / Renin receptor Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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EB06118 1
EB06118 (2µg/ml) of Mouse Kidney lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB06118 2
EB06118 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ELDF10, HT028, MGC99577, MRXE, MSTP009, XMRE, ATPase, H+ transporting, lysosomal interacting protein 2, embryonic liver differentiation factor 10
Official Symbol: ATP6AP2
Accession Number(s): NP_005756.2
Human GeneID(s): 10159
Non-Human GeneID(s): 70495 (mouse)

Immunogen

Peptide with sequence C-SIIYRMTNQKIRMD, from the C Terminus of the protein sequence according to NP_005756.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1). An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2µg/ml. Primary incubation 1 hour at room temperature.
IHC: This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6(1):9-19, PMID: 27956412.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Rat

Specific References

This antibody has been successfully used in IHC in Human:
Sarah J Delforce, Eugenie R Lumbers, Celine Corbisier de Meaultsart, Yu Wang, Anthony Proietto, Geoffrey Otton, Jim Scurry, Nicole M Verrills, Rodney J Scott and Kirsty G Pringle.
Expression of renin–angiotensin system (RAS) components in endometrial cancer.
Endocr Connect. 2017 Jan;6(1):9-19.
PMID: 27956412

This antibody has been successfully used in IHC in Human:
Pringle KG, Zakar T, Yates D, Mitchell CM, Hirst JJ, Lumbers ER.
Molecular evidence of a (pro)renin/(pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2.
J Renin Angiotensin Aldosterone Syst. 2010 Aug 11.
PMID: 20702505

This antibody has been successfully used in Rat in the following paper:
Zhu A, Yoneda T, Demura M, Karashima S, Usukura M, Yamagishi M, Takeda Y.
Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats.
J Hypertens. 2009 Apr;27(4):800-5.
PMID: 19516179

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