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Goat Anti-Arylsulfatase A Antibody (EB07457)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07457 | Goat Anti-Arylsulfatase A Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Mouse | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$95.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected])
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfataseOfficial Symbol: ARSA
Accession Number(s): NP_000478.2
Human GeneID(s): 410
Non-Human GeneID(s): 11883 (mouse), 315222 (rat)
Immunogen
Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 53.6kDa according to human NP_000478.2 and 53.7kDa according to Mouse NP_033843.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.
Species Reactivity
Tested: MouseExpected from sequence similarity: Human, Mouse, Rat
Specific References
This antibody has been successfully used in the following paper:
Kanbe H, Kamijo Y, Nakajima T, Tanaka N, Sugiyama E, Wang L, Fang ZZ, Hara A, Gonzalez FJ, Aoyama T.
Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice.
Arch Toxicol. 2014 Feb;88(2):367-79.
PMID: 24065054
This antibody has been successfully used in WB on Mouse in the following paper:
Kimura T, Nakajima T, Kamijo Y, Tanaka N, Wang L, Hara A, Sugiyama E, Tanaka E, Gonzalez FJ, Aoyama T.
Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice.
PPAR Res. 2012;2012:174932.
PMID: 22645601
This antibody has been successfully used in WB on Mouse in the following paper:
Kiebish MA, Young DM, Lehman JJ, Han X.
Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1α-/- mouse cortex: a potential lipidomic role of PGC-1α in neurodegeneration.
J Lipid Res. 2012 Feb;53(2):273-81.
PMID: 22114039
This antibody has been successfully used in the following paper:
Zhang X, Nakajima T, Kamijo Y, Li G, Hu R, Kannagi R, Kyogashima M, Aoyama T, Hara A.
Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides.
Biochem Biophys Res Commun. 2009 Dec 25;390(4):1382-8.
PMID: 19895791