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Code Name Applications Availability Size Tested species Price Grade  
EB07748 Goat Anti-ACACB Antibody Pep-ELISA, EIA Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

OR

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07748 1
EB07748 (1.5ug/ml) as the reporter with EB002020 as the capture rabbit antibody (5ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ACACB, acetyl-Coenzyme A carboxylase beta, ACCB, HACC275, acetyl-CoA carboxylase 2
Official Symbol: ACACB
Accession Number(s): NP_001084.2
Human GeneID(s): 32

Immunogen

Peptide with sequence C-QLGEPDLSDKDRKD, from the internal region of the protein sequence according to NP_001084.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277kDa according to NP_001084.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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